Primary human cholangiocytes are specialized epithelial cells lining the bile ducts, playing a vital role in bile modification, secretion, and liver homeostasis. Supplied in a cryopreserved format with high post-thaw viability, these cells retain key functional markers, making them ideal for studies in cholangiopathies, liver fibrosis, bile duct biology, and drug toxicity. Suitable for 2D and 3D culture systems, they provide a physiologically relevant model for investigating biliary diseases and testing therapeutic interventions.